重组人PD-L1蛋白-ACROBiosystems百普赛斯

Human PD-L1 / B7-H1 Protein, His Tag (MALS verified)

分子别名（Synonym）

PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

表达区间及表达系统（Source）

Human PD-L1, His Tag (PD1-H5229) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1 ).

Predicted N-terminus: Phe 19

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.0 kDa. The protein migrates as 30-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>98% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景（Background）

程序性细胞死亡1配体1（PDL1）也称为B7-H、B7H1、MGC142294、MGC1422 96、PD-L1、PDCD1L1和PDCD1LG1，是生长中的B7免疫分子家族的成员，参与细胞和体液免疫反应的调节。PDL1是一个细胞表面免疫球蛋白超家族，在细胞外区域内有两个Ig样结构域和一个短的细胞质结构域。这种蛋白质在大多数外周组织以及造血细胞中广泛表达。PDL1及其属于CD28分子家族的受体之间的相互作用在调节T细胞活化和耐受中提供刺激和抑制信号。PDL1可以通过诱导细胞凋亡和阻止细胞周期进展来抑制正在进行的T细胞反应。