7385-67-3
基本信息
尼罗红
NILE BLUE A OXAZONE
NILE RED
9-(diethylamino)-5h-benzo[a]phenoxazin-5-on
nile
9-(diethylamino)benzo[a]phenoxazin-5(5H)-one
FLUORESCENCE QY-STANDARD E CERTIFIED
Fluorescent Spectral Standard E, certified
9-(Diethylamino)benzo[a]phenoxazin-5(5H)-on
Nile Red, pure, 99%
9-Diethylamino-benzo[a]phenoxazin-5-one
7-(Diethylamino)-1,2-[1,3]butadieno-3H-phenoxazine-3-one
9-Diethylamino-5H-benzo[a]phenoxazine-5-one
Oxazine O-17M
Phenoxazone
物理化学性质
| 熔点 | 203-205 °C(lit.) |
| 熔点 | 203-205 °C(lit.) |
| 沸点 | 457.53°C (rough estimate) |
| 密度 | 1.1871 (rough estimate) |
| 折射率 | 1.5700 (estimate) |
| 储存条件 | 2-8°C |
| 储存条件 | room temp |
| 溶解度 | 在甲醇中可溶1mg/mL |
| 酸度系数(pKa) | 4.08±0.20(Predicted) |
| 形态 | 粉末 |
| 颜色 | 深红色至栗色 |
| BRN | 279110 |
| 稳定性 | 稳定的。与强氧化剂不相容。 |
| InChI | InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 |
| InChIKey | VOFUROIFQGPCGE-UHFFFAOYSA-N |
| SMILES | C12=CC=CC=C1C(=O)C=C1C2=NC2=C(C=C(N(CC)CC)C=C2)O1 |
| CAS 数据库 | 7385-67-3(CAS DataBase Reference) |
| EPA化学物质信息 | 5H-Benzo[a]phenoxazin-5-one, 9-(diethylamino)- (7385-67-3) |
安全数据
| 危险性符号(GHS) |  GHS07 |
| 警示词 | 警告 |
| 危险性描述 | H302-H315-H319-H335 |
| 防范说明 | P261-P280-P301+P312-P302+P352-P305+P351+P338 |
| 安全说明 | 24/25 |
| 安全说明 | S24/25 |
| WGK Germany | 3 |
| WGK Germany | 3 |
| F | 8-10 |
| 海关编码 | 29349990 |
应用领域
知名试剂公司产品信息
Nile Red, pure, 99%(7385-67-3) Acros Organics
Nile Red(7385-67-3) TCI Shanghai
常见问题列表
| Target | Value |
|
lipid droplet
() |
Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments.
When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.