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网站主页 化工产品目录 生物化工 核苷类药物 核苷酸及其类似物 5-氟脲嘧啶 5-氟脲嘧啶
  • 5-氟脲嘧啶|T0984|TargetMol

5-氟脲嘧啶|T0984|TargetMol

5-Fluorouracil
￥291 200mg 起订
上海 更新日期：2024-09-24

TargetMol中国（陶术生物）

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邮箱：marketing@targetmol.com

产品详情:

中文名称：
5-氟脲嘧啶
英文名称：
5-Fluorouracil
CAS号：
51-21-8
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
99.98%
产品类别：
抑制剂
货号：
T0984

Product Introduction

Bioactivity

名称5-Fluorouracil
描述5-Fluorouracil (5-FU) is a uracil analog, an inhibitor of DNA synthesis. 5-Fluorouracil has antitumor activity and affects pyrimidine synthesis through inhibition of thymidylate synthase. 5-Fluorouracil causes apoptosis and autophagy.
细胞实验After a 7-day habituation period, the mice were divided into three groups (vehicle group, dextrin group, and ED group; n = 6 mice per group) that had the same mean body weight (time of grouping was designated as day 0). Then, mice were treated by tail vein injections from day 0 to 4; mice in the dextrin and ED groups received 40 mg/kg/day of 5- fluorouracil (5-FU) injection 250 mg, while mice in the vehicle group received 10 mL/kg/day physiological saline, which was equivalent to the dose of 5-FU. Additionally, twice a day from day 0 to 6, ED group mice received 1.6 kcal/0.8 mL/day ED administered orally, while mice in the vehicle and dextrin groups received dextrin containing the same amount of calories. Body weight and food consumption were measured before administration of 5-FU and ED on days 0 and 7. Food consumption was measured with respect to each group. Mice were accommodated individually in specially prepared polycarbonate cages, and two or more fresh stools per mouse were scored as follows: 0, the stools were not crushed when pushed by human fingers; 1, the stools were crushed, but the core remained when pushed by human fingers; 2, the stools were crushed and the core did not remain when pushed by human fingers; 3, the stools were crushed and stuck to the fingers when pushed by human fingers; 4, the stools lost their shape just from being touched. Autopsies were conducted on day 7. After bleeding, the large intestine (the colon and rectum) was taken, and the length was measured. The lumen was washed with physiological saline, the excess water was wiped off, and specimens were weighed. Section 3 cm distal from the center of the large intestine was fixed with formalin for histological evaluation. Salivary glands (the submandibular gland and sublingual gland) were collected and weighed. The collected salivary glands were fixed with formalin, and after the tissue sections were prepared, they were stained with hematoxylin and eosin [3].
体外活性方法：人心肌细胞 HCM、人脐静脉内皮细胞 HUVE 和 人结肠癌细胞 HCT116、HT29 用 5-Fluorouracil (0.01-1000 µM) 处理 24-96 h，使用 MTT 方法检测细胞生长抑制情况。 结果：72 h 时 5-Fluorouracil 对 HCM、HUVE、PHCT116 和 HT29 细胞的 EC50 分别为 4.866 μM、3.832 μM、13.72 μM 和 106.8 μM。[1] 方法：平滑肌细胞用 5-Fluorouracil (0.05-10 mM) 处理 24 h，使用 Flow Cytometry 方法检测细胞凋亡情况。 结果：浓度为 0.1、1 和 10mM 的 5-Fluorouracil 在处理 24 h 后诱导培养的平滑肌细胞凋亡，并且凋亡细胞从培养皿中分离。[2] 方法：人结肠癌细胞 SW620 用 5-Fluorouracil (13 μg/mL) 孵育 24-48 h，使用 ALP detection kit 检测 ALP 活性。 结果：碱性磷酸酶 (ALP) 已被用于监测某些抗癌化合物的分化效果。未处理的 SW620 细胞表现出相对较低的 ALP 活性，而在用 13μg/ml 5-FU 处理的细胞中，ALP 的活性以时间依赖的方式达到高水平。[3]
体内活性方法：为检测体内抗肿瘤活性，将 5-Fluorouracil (10-40 mg/kg) 腹腔注射给携带小鼠腹水肝癌肿瘤 H22 的小鼠，每天一次，持续十天。 结果：10 mg/kg 的 5-Fluorouracil 抑制肿瘤生长，同时维持小鼠的免疫功能。5-Fluorouracil 可发挥低剂量、低毒的抗肿瘤作用，刺激宿主免疫系统。[4] 方法：为研究 5-Fluorouracil 诱导的肠道损伤，将 5-Fluorouracil (100-200 mg/kg) 单次腹腔注射给 BALB/c 小鼠。 结果：5-Fluorouracil 治疗的动物的体重及腹泻症状以剂量依赖的方式显著降低。5-Fluorouracil 治疗组的 occludin 和 claudin-1 蛋白的表达显著降低。5-Fluorouracil 治疗组 NF-κBp65 蛋白和 TNF-α mRNA 的表达明显高于对照组。[5]
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度10% DMSO+90% Saline : 1.3 mg/mL (9.99 mM), Working solution is recommended to be prepared and used immediately.
Ethanol : 1.3 mg/mL (10 mM)
H2O : 10 mg/mL (76.8 mM), Heating at 50℃ is recommended.
DMSO : 18.33 mg/mL (140.94 mM)
关键字5 Fluorouracil | Endogenous Metabolite | inhibit | Nucleoside Antimetabolite/Analog | NSC19893 | Apoptosis | Inhibitor | 5Fluorouracil | Human immunodeficiency virus | 5-Fluorouracil | HIV | NSC-19893
相关产品Guanidine hydrochloride | Daidzein | Glycerol | Emtricitabine | Sucrose | Ferulic Acid | Lamivudine | Thymidine
相关库抑制剂库 | 经典已知活性库 | 抗癌上市药物库 | 抗癌活性化合物库 | 已知活性化合物库 | 抗衰老化合物库 | FDA 上市药物库 | 抗病毒库 | 药物功能重定位化合物库 | 抗癌药物库
5-FU|||Fluorouracil|||5-氟脲嘧啶|||NSC 19893|||5-Fluoracil|||氟尿嘧啶|TargetMol

公司简介

上海陶术生物科技有限公司为美国Target Molecule Corp. ( Target Mol ) 在上海建立的全资子公司。我们与美国波士顿、德国慕尼黑的同事一起，为北美、欧洲和亚洲从事药物研发和生物学研究的科学家提供优质的产品和专业的服务。公司下设筛选事业部，化学事业部，生物事业部和新材料部。 从虚拟筛选到实体化合物分子供应；从商业化产品销售到个性化定制合成；从对明确靶点的分子筛选到对明确分子的多靶点筛选，从高通量筛选到化学结构优化，我们都可以满足您的科研用品及技术服务的需求。 经过在中国市场五年的精心耕耘，我们已成为筛选化合物领域优秀的供应商，为超过五百家学校和各类企业提供了品质卓越的小分子化合物和药物筛

成立日期 (12年)
注册资本 566.2651万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,试剂,定制,服务
主营行业 化学试剂,生物活性小分子

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