CTAB植物基因组DNA快速提取试剂盒

产品介绍：

     改进的经典CTAB植物DNA抽提液内（添加多种针对植物特点的多糖、多酚去除成份）迅速裂解细胞和灭活细胞内核酸酶，氯仿抽提后通过离心**多糖、多酚和蛋白质（根据需要，上清中还加入异丙醇离心沉淀基因组DNA，进一步去除其它各种杂质），然后基因组DNA在高离序盐状态下选择性吸附于离心柱内硅基质膜，再通过一系列快速的漂洗－离心的步骤， 进一步将多糖、多酚和细胞代谢物、蛋白等杂质去除，**低盐的洗脱缓冲液将纯净基因组DNA从硅基质膜上洗脱。

产品特点：

1.离心吸附柱内硅基质膜全部采用进口特制吸附膜，柱与柱之间吸附量差异极小，可重复性好。克服了国产试剂盒膜质量不稳定的弊端。

2.不需要使用有毒的苯酚等试剂，也不需要乙醇沉淀等步骤。

3.快速，简捷，单个样品操作一般可在1小时内完成。

4.数种去多糖、多酚成份和多次柱漂洗确保高纯度，OD260/OD280典型的比值达1.7～1.9，长度可达30 kb -50kb，可直接用于PCR，Southern-blot和各种酶切反应。

 使用该产品发表的部分文章：

1.Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, DOI 10.1007/s00299-013-1413-6

2.Taxonomy and phylogeny of Ceriporia (Polyporales, Basidiomycota) with an emphasis of Chinese collections. Mycol Progress 2014, 13: 81–93

3.Phlebiporia bubalina gen. et. sp. nov. (Meruliaceae, Polyporales) from southwest China with a preliminary

4.Down-Regulated miR-30a in Clear Cell Renal Cell Carcinoma Correlated with Tumor Hematogenous Metastasis by Targeting Angiogenesis-Specific DLL4. miR-30a Targets DLL4 in RCC Metastasis. June 2013 | Volume 8 | Issue 6 | e67294

5.A preliminary report on decay and canker of Acacia richii caused by Inonotus rickii in China. For. Path. 44 (2014) 82–84 . doi: 10.1111/efp.12088. 2014 Blackwell Verlag GmbH.

6.Hispidaedalea gen. nov. and Griseoporia taiwanense sp. nov. (Gloeophyllales, Basidiomycota) based on morphological and molecular characters. ORIGINAL ARTICLE. Mycol Progress. DOI 10.1007/s11557-014-0966-2.

7.Two new species of the Heterobasidion insulare complex based on morphology and molecular data. Mycoscience 2014, 55(4): 289–298

8.Three homologous genes encoding functional D8-sphingolipid desaturase in Populus tomentosa. Genes Genom 2014, 36: 293–301

9.Isolation, Characterization, and Structure Analysis of a Vacuolar Processing Enzyme Gene (MhVPEγ) from Malus hupehensis (Pamp) Rehd. Appl Biochem Biotechnol 2014, 73: 579–5954

10.Morphological characters and molecular data reveal a new species of Fomitopsis (Polyporales) from southern China. Mycoscience 2014, http://dx.doi.org/10.1016/j.myc.2014.05.0044

11.Morphology and molecular phylogeny for two new species of Fomitopsis (Basidiomycota) from South China. Mycol Progress 2014, 13: 905–914

12.Coltriciella globosa and C. pseudodependens spp. nov. (Hymenochaetales) from southern China based on morphological and molecular characters. Mycoscience 2014, http://dx.doi.org/10.1016/j.myc.2014.06.001

13.Contribution of arbuscular mycorrhizal fungi of sedges to soil aggregation along an altitudinal alpine grassland gradient on the Tibetan Plateau. Environmental Microbiology 2015, (17)8:2841–2857

14.Stability Assay of Three DNA Extraction Methods for Detection of Strawberry Vein Banding Virus. Acta Agriculturae Boreali-Sinica 2015, 30(3):200-204