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  • 组织/细胞RNA快速提取试剂盒

组织/细胞RNA快速提取试剂盒

Tissue/Cell RNA Rapid Extraction Kit
1200 1盒 起订
浙江 更新日期:2024-07-12

杭州昊鑫生物科技股份有限公司

非会员
联系人:谢美姿
电话:15906629305拨打
手机:15906629305 拨打
邮箱:351778830@qq.com

产品详情:

中文名称:
组织/细胞RNA快速提取试剂盒
英文名称:
Tissue/Cell RNA Rapid Extraction Kit
品牌:
艾德莱
产地:
北京
保存条件:
常温
纯度规格:
97
产品类别:
RNA提取试剂盒
别名:
组织/细胞RNA快速提取试剂盒
分子式:
货号:
RN2802
用途范围:
适用于快速提取普通动物和易裂解动物组织总RNA,使用独有基因组DNA清除柱技术确保有效清除gDNA残留,不需要使用DNase消化,RNA可直接用于反转录荧光定量PCR.,Northern-blot等下游实验。
规格:
50次
是否进口:

RN28-EASYspin Plus 组织/细胞RNA快速提取试剂盒

货号#
规格
价格
RN2802
50 
1200.00 

产品介绍:

     本公司独家推出EASYspin无苯酚、氯仿RNA快速提取技术基础上,又独家研发成功基因组DNA清除柱技术确保有效清除gDNA残留,得到的RNA不需要DNase消化,可直接用于PCR、荧光定量PCR等实验。独特的裂解液迅速裂解细胞和灭活细胞RNA酶,然后裂解混合物通过一个基因组DNA清除柱,基因组DNA被清除而RNA穿透滤过。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。 
 

产品特点:

1.完全不使用有毒的苯酚,氯仿,beta巯基乙醇等试剂,也不需要乙醇沉淀等步骤。 
2.快速,简捷,单个样品操作一般可在30分钟内完成。 
3.独家研发成功基因组DNA清除柱技术确保有效清除gDNA残留,得到的RNA不需要DNase消化,可直接用于PCR、荧光定量PCR等实验。 
4.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.0,基本无DNA残留,可用于RT-PCR,Northern-blot和各种实验。

使用该产品发表的部分文章

  1.  Engagement of Soluble Resistance-Related Calcium Binding Protein (sorcin) with foot-and-mouth disease virus (FMDV) VP1 inhibits type I interferon response in cells. Available online 21 May 2013

  2. Overexpression of small heat shock protein 21 protects the Chinese oak 4 silkworm Antheraea pernyi against thermal stress. Journal of Insect Physiology, IP 3099 No. of Pages 7, Model 5G 14 June 2013

  3. S-Adenosylmethionine-induced adipogenesis is accompanied by suppression of Wnt/b-catenin and Hedgehog signaling pathways. Mol Cell Biochem (2013)382:59–73,DOI 10.1007/s11010-013-1718-3

  4. A critical role of interferon-induced protein IFP35 in the type I interferon response in cells induced by foot-and-mouth disease virus (FMDV) protein 2C. Archives of Virology 2014, DOI 10.1007/s00705-014-2147-7

  5. Saikosaponin-D Attenuates Heat Stress-Induced Oxidative Damage in LLC-PK1 Cells by Increasing the Expression of Anti-Oxidant Enzymes and HSP72. The American Journal of Chinese Medicine, 2014, 42(5): 1–17

  6. The Cellular Prion Protein Negatively Regulates Phagocytosis and Cytokine Expression in Murine Bone Marrow-Derived Macrophages. PLoS ONE 2014, 9(7): e102785. doi:10.1371/journal.pone.0102785

  7. Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth. Archives of Virology 2015, 160:1449–1461

  8. Baicalin protects sertoli cells from heat stress-induced apoptosis viaactivation of the Fas/FasL pathway and Hsp72 expression. Reproductive Toxicology 2015, Accepted 12 June 2015

  9. Identification and expression of an ecdysteroid-responsive amylase from red crayfish Procambarus clarkii. Fisheries Science 2015, 81(2): 345-352

  10. Small Heat Shock Proteins and Eicosanoid Pathways Modulate Caspase-1 Activity in the Fat Bodies of Antheraea pernyi. first posted online September 3, 2015; doi: http://dx.doi.org/10.1101/026039

  11. Inflammes-dependent regulation of IL-1b secretion induced by the virulent Mycobacterium bovis Beijing strain in THP-1 macrophages.Antonie van Leeuwenhoek 2015, DOI 10.1007/s10482-015-0475-6

  12. Baicalin protects sertoli cells from heat stress-induced apoptosis via activation of the Fas/FasL pathway and Hsp72 expression. Reproductive Toxicology 2015, 57: 196-203

  13. Plumbagin Ameliorates CCl4-Induced Hepatic Fibrosis in Rats via the Epidermal Growth Factor Receptor Signaling Pathway. Evidence-Based Complementary and Alternative Medicine 2015, http://dx.doi.org/10.1155/2015/645727

  14. Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol. Oxidative Medicine and Cellular Longevity 2016, Article ID 2572175, 15 pages http://dx.doi.org/10.1155/2016/2572175

  15. Metabonomics applied in exploring the antitumour mechanism of physapubenolide on hepatocellular carcinoma cells by targeting glycolysis through the Akt-p53 pathway. Scientific Reports 2016, 6:29926 | DOI: 10.1038/srep29926

  16. Hesperidin prevents lipopolysaccharide-induced endotoxicity in rats.I mmunopharmacology And Immunotoxicology 2016, 38(5): 364–371

  17. A study of the damage of the intestinal mucosa barrier structure and function of Ctenopharyngodon idella with Aeromonas hydrophila. Fish Physiology and Biochemistry 2017, 43(5): 1223–1235

  18. Both point mutations and low expression levels of the nicotinic acetylcholine receptor β1 subunit are associated with imidacloprid resistance in an Aphis gossypii (Glover) population from a Bt cotton field in China. Pesticide Biochemistry and Physiology 2017, 141: 1-8

  19. Silencing of histone methyltransferase NSD3 reduces cell viability in osteosarcoma with induction of apoptosis. Oncology Reports 2017, 2796-2802

  20. Acute aflatoxin B1 – Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein metabolism in rats. Toxicology Reports 2017, 4: 408-414(出口产品发的文章)

  21. Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus. Acta Pharmaceutica Sinica B 2017, 3: 373-380

  22. Structural analysis and immunomodulatory effect of polysaccharide from Atractylodis macrocephalae Koidz. on bovine lymphocytes. Carbohydrate Polymers 2017, 174: 1213-1223

  23. Transcriptome profiling of spleen provides insights into the antiviral mechanism in Schizothorax prenanti after poly (I: C) challenge. Fish & Shellfish Immunology 2017, 62: 13-23

  24. Front. Microbiol., 19 July 2017 | https://doi.org/10.3389/fmicb.2017.01351

  25. VP2 of Infectious Bursal Disease Virus Induces Apoptosis via Triggering Oral Cancer Overexpressed 1 (ORAOV1) Protein Degradation. Front. Microbiol. 2017

  26. MicroRNA-20a contributes to cisplatin-resistance and migration of OVCAR3 ovarian cancer cell line. Oncology letters. 2017, 1780-1786  

  27. Down-regulation of aquaporin 5-mediated epithelial-mesenchymal transition and anti-metastatic effect by natural product Cairicoside E in colorectal cancer. Molecular Carcinogenesis 2017, 11 September 2017 DOI: 10.1002/mc.22712  View/save citation

  28. The impact of Zearalenone on the meiotic progression and primordial follicle assembly during early oogenesis. Toxicology and Applied Pharmacology 2017, 329: 9-17

  29. Benzo[a]pyrene exposure under future ocean acidification scenarios weakens the immune responses of blood clam, Tegillarca granosa. Fish & Shellfish Immunology 2017, 63: 465-470

  30. Transcriptome analysis of sex-related genes in the blood clam Tegillarca granosa. PLoS ONE 12(9): e0184584

  31. Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway. Front. Cell. Infect. Microbiol. 2017, | https://doi.org/10.3389/fcimb.2017.00323


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公司简介

杭州昊鑫生物科技股份有限公司成立于2009年,产品和服务涵盖生命科学研究技术的诸多方面,提供覆盖分子生物学、细胞生物学、植物学、生物化学、蛋白组学、免疫学等领域的实验产品以及生物技术服务等。目前一级代理品牌有:MCE(Medchemexpress)、Biochannel、AATbio、invivogen、Abnova、Atlas、Origene、Biovision、云克隆(Cloud-clone)、艾德莱(Aidlab)、Cayman、Jackson、Epigentek、Prospec、Sciencell、icellbioscience(赛百慷)、hkABCbio、chondrex、Mybiosource、Abbexa、Innovrsrch、HPI、Hitobiotec、Greerlabs,Ostex,4ADI,LDN等。

成立日期 (16年)
注册资本 500万元
员工人数 10-50人
年营业额 ¥ 100万-300万
经营模式 贸易,试剂,服务
主营行业 化学试剂

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