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  • 淀粉样前体蛋白(APP)重组蛋白 生产供应商 艾普蒂生物
  • 淀粉样前体蛋白(APP)重组蛋白 生产供应商 艾普蒂生物

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淀粉样前体蛋白(APP)重组蛋白 生产供应商 艾普蒂生物

Recombinant human APP protein
询价 50μg 起订
50μg 起订
湖北 更新日期:2024-09-05

湖北艾普蒂生物工程有限公司

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产品详情:

中文名称:
淀粉样前体蛋白(APP)重组蛋白
英文名称:
Recombinant human APP protein
品牌:
艾普蒂
产地:
湖北
保存条件:
-20℃
产品类别:
重组蛋白
货号:
PA1000-201
用途范围:
仅供科研使用
规格:
50μg 100μg 1mg

产品名称

人APP 蛋白knockout HEK-293T cell line

Parental Cell Line

HEK293T

Organism

Human

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5

Passage number

<20

Knockout validation

Immunocytochemistry (ICC), Sanger Sequencing, Western Blot (WB)

经测试应用

适用于: WB, ICCmore details

Biosafety level

2

常规说明

Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.


Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.


Culture medium: DMEM (High Glucose) + 10% FBS


Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.


1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.

2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.

3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.

4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.


Subculture guidelines:


All seeding densities should be based on cell counts gained by established methods.

A guide seeding density of 2x104 cells/cm2 is recommended.

A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required.

Cells should be passaged when they have achieved 80-90% confluence.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.


淀粉样前体蛋白;APP protein;APP重组蛋白;

公司简介

湖北艾普蒂生物工程有限公司(简称:艾普蒂)由在国内科研试剂领域有着十几年从业经验的专业技术团队和企业管理团队组建而成,专门从事以抗体、蛋白、细胞、化学品为核心的试剂产品研发与销售。 我们长期聚焦于生命科学、生物医药、医疗诊断、分析检测等领域,专注于为生命科学和生物技术领域的客户提供优质的产品和技术服务。提供生命科学 试剂、供应链服务等,为更多的生命科学科研工作者提供 专业、全面、安全、快捷的产品和服务。产品覆盖 基因、蛋白、细胞、组织、动物等水平的各种研究。 致力于成长为我国重要的生命科学试剂和成果转化一体化的领导者、致力于成为中国大型的科研试剂产品供应商之一。

成立日期 (4年)
注册资本 600万人民币
员工人数 1-10人
年营业额 ¥ 500万-1000万
经营模式 贸易,工厂,试剂
主营行业 化学试剂,医药原料,诊断试剂

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