重组蛋白A

(1)非抗体柱纯化，产品中无任何其它抗体如IgG等的污染。 (2)pH耐受范围广，可耐受0.5 M NaOH和0.5M HCl 处理，不降解，抗体结合能力不变。 (3)产品有特为抗体纯化柱设计的连接基团，可直接用于蛋白A柱的制备。 (4)具有生产规模大，质量稳定，纯度高等优点。 重组蛋白A可偶联到固体分离介质（如agarose）上，用于单克隆抗体或多克隆抗体的纯化。重组蛋白A可与多种报告分子（荧光染料、酶标记、生物素、胶体金、放射性标记等）偶联并不影响其与抗体结合的活性。这些偶联后的蛋白A衍生物可用于Western-blot、ELISA、免疫组化等的抗体检测。

The recombinant Protein A is a genetically engineering protein containing IgG-binding domains.Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b,IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonaland polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical tests.