DTT is widely used as reductant, and sustains stability of the enzyme reducing
1. Molecular biology.
2. Excellent reagent for maintaining the ?SH group in a reduced state; quantitative reduction of disulfide; DTT can effectively
reduce protein disulfide bonds in sample buffer before SDS-PAGE; DTT can also be used for reduction cross-linking Disulfide bridge
of N,N'-bis(acryloyl)cystamine to decompose polyacrylamide gel matrix; used in protein sequence analysis to remove disulfide
linkages; to investigate increased and dynamic reduction Reaction; prevents oxidation reactions that occur when cholecystokinin is
extracted from the brain and duodenal tissues.

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