MHY1485

Description

MHY1485 is a cell-permeable activator of mTOR that has been shown to increase levels of cellular mTOR Ser²⁴⁴⁸ and downstream substrate 4E-BP Thr^37/46 phosphorylation in rat liver Ac2F cells. It can induce cellular LC3-II accumulation in Ac2F cells and has been shown to inhibit autophagy by suppressing the fusion between autophagosomes and lysosomes.

Uses

MHY1485 has been used:

• to study the effect of mammalian target of rapamycin mTOR signalling on in vitro O-GlcNAcylation
• to inhibit autophagy
• as a mTOR agonist to demonstrate that the O-linked N-acetylglucosamine transferase- RNA helicase p68 (OGT-DDX5) axis regulates colorectal cancer cell proliferation and metastasis

Biochem/physiol Actions

MHY1485 is mTOR activator that potently inhibits autophagy by suppression of fusion between autophagosomes and lysosomes.

References

1) Choy et al. (2012), Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion; PLoS One, 7(8) e43418 2) Cheng et al. (2015), Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators; PLoS One, 10(2) e0117769 3) Zhao et al. (2016), MHY1485 activates mTOR and protects osteoblasts from dexamethasone; Biochem. Biophys. Res. Commun., 481 212 4) Yang et al. (2017), MHY1485 ameliorates UV-induced skin cell damages via activating mTOR-Nrf2 signaling; Oncotarget, 8 12775 5) Li and Siragy (2015), (Pro)renin receptor regulates autophagy and apoptosis in podocytes exposed to high glucose; J. Physiol. Endocrinol. Metab., 309 E302