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Bathocuproinedisulfonic acid disodiuM salt hydrate cna be usd as reagent used for determination of Cu1 and Cu-protein complexes.2

Bathocuproinedisulfonic acid disodium salt hydrate was used as chelator in electron spin resonance spin trapping technique to study free radical generation in rats with acute sodium formate poisoning. It was used as reagent for determination of Cu and Cu-protein complexes.

Bathocuproine disulfonic acid (BCDS) is a stable Cu⁺ chelator and BCDS-Cu⁺ inhibits the HIV-1 wild type protease as well as a mutant HIV-1 protease lacking cysteine. It is an extracellular copper chelator.